Composite

Part:BBa_K5217033:Design

Designed by: CHUQIAO LIN   Group: iGEM24_BGI-MammothEdu-East   (2024-09-26)


pINO1_AmMYS_TEF1t


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1015
    Illegal SpeI site found at 2056
    Illegal PstI site found at 179
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2056
    Illegal PstI site found at 179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1717
    Illegal BglII site found at 2066
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1015
    Illegal SpeI site found at 2056
    Illegal PstI site found at 179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1015
    Illegal SpeI site found at 2056
    Illegal PstI site found at 179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed to express terpene compounds in Saccharomyces cerevisiae



Source

plasmid


References